Calculate the insert volume for In-Fusion reaction

Calculate the insert volume for In-Fusion reaction

calc_InFusion_insert_volume(
  seq_i,
  seq_v,
  conc_i,
  conc_v,
  uL_v,
  ratio_i = 2,
  ratio_v = 1
)

Arguments

seq_i

DNA sequence of insertion
seq_v

DNA sequence of vector
conc_i

DNA concentration (ng/uL) of purified insertion
conc_v

DNA concentration (ng/uL) of purified vector
uL_v

Supplied volume (uL) of purified insertion
ratio_i

Molecular ratio of insertion
ratio_v

Molecular ratio of vector

Examples

calc_InFusion_insert_volume(
  seq_i = dstr_rand_seq(1, 1000, seed = 1),
  seq_v = dstr_rand_seq(1, 5000, seed = 1),
  conc_i = 5, conc_v = 30,
  uL_v = 1.25
)
#> # A tibble: 2 x 7
#>   seq_name num_bases applox_MW  conc sup_uL sup_ng sup_nmol
#>   <chr>        <int>     <dbl> <dbl>  <dbl>  <dbl>    <dbl>
#> 1 insert        1000   607558.     5   3.00   15.0   24694.
#> 2 vector        5000  3037158.    30   1.25   37.5   12347.